• Gordana Đurić Genetic Resources Institute, University of Banja Luka Faculty of Agriculture, University of Banja Luka
• Biljana Lolić Faculty of Agriculture, University of Banja Luka
• Mirela Kajkut Zeljković Genetic Resources Institute, University of Banja Luka
• Duška Delić Faculty of Agriculture, University of Banja Luka
• Mirjana Koprivica Faculty of Agriculture, University of Banja Luka
• Mariana Radulović Faculty of Agriculture, University of Banja Luka
• Petar Nikolić Faculty of Agriculture, University of Banja Luka
• Nikola MićićGenetic Resources Institute, University of Banja Luka Faculty of Agriculture, University of Banja Luka
• Živojin Erić Genetic Resources Institute, University of Banja Luka Faculty of Science, University of Banja Luka

Abstract

Detection of viruses presence were carried out for the 225 a of pome and stone fruit trees from the collection of the Genetic Resources Institute of University of Banja Luka, located within the Botanical Garden of the University, tested by DAS-ELISA. The pome fruit trees were analyzed on presence of the following viruses: Apple Chlorotic Leaf Spot Virus (ACLSV), Apple Stem Grooving Virus (ASGV), Apple Stem Pitting Virus (ASPV) and Apple Mosaic Virus (ApMV). The stone fruits were analyzed on presence of Plum Pox Virus (PPV), Prune Dwarf Virus (PDV) and Prunus Necrotic Ring Spot Virus (PNRSV). All samples were tested serologically by DAS-ELISA. In addition to this, virus negative pear and apple accessions were tested for 'Candidatus Phytoplasma mali' and 'Candidatus Phytoplasma pyri' presence using nested-PCR/RFLP analyses.